Fluorescent transmembrane anion transporters: shedding light on anionophoric activity in cells
نویسندگان
چکیده
Chemistry, University of Southampton, Sou [email protected]; Tel: +44 (0)23 8059 3332 Department of Pathology and Experimen Research Group, University of Barcelona, Ba Singapore Bioimaging Consortium, Agency (A*STAR), Singapore 138667, Singapore Department of Chemistry and MedChem Pr University of Singapore, Singapore 117543, † Electronic supplementary information details and characterisation of all com studies, stacked spectra and tted bindin spectroscopy studies data; various vesicle transport studies; details of MTT assay curves and IC50 values for cell viabi microscopy images and co-staining expe crystallographic data in CIF or o 10.1039/c6sc01643j ‡ The data underlying this paper h http://dx.doi.org/10.5258/SOTON/392749 policy. Cite this: DOI: 10.1039/c6sc01643j
منابع مشابه
Structure–Activity Relationships in Tripodal Transmembrane Anion Transporters: The Effect of Fluorination
A series of easy-to-make fluorinated tripodal anion transporters containing urea and thiourea groups have been prepared and their anion transport properties studied. Vesicle anion transport assays using ion-selective electrodes show that this class of compound is capable of transporting chloride through a lipid bilayer via a variety of mechanisms, including chloride/H(+) cotransport and chlorid...
متن کاملDoes lipophilicity affect the effectiveness of a transmembrane anion transporter? Insight from squaramido-functionalized bis(choloyl) conjugates.
Six squaramido-functionalized bis(choloyl) conjugates were synthesized and fully characterized on the basis of NMR ((1)H and (13)C) and ESI MS (LR and HR) data. Their transmembrane anionophoric activity was investigated in detail by means of chloride ion selective electrode technique and pyranine assay. The data indicate that this set of compounds is capable of promoting the transmembrane trans...
متن کاملThiourea isosteres as anion receptors and transmembrane transporters.
Compounds containing cyanoguanidine and 3-amino-1,2,4-benzothiadiazine-1,1-dioxide have been studied as anion receptors and transporters. Significant affinity for oxo-anions was observed in organic solution and the receptors were found to function as transmembrane chloride/nitrate antiporters with transport rates enhanced in the presence of valinomycin-K(+) complex.
متن کاملMacrophages possess probenecid-inhibitable organic anion transporters that remove fluorescent dyes from the cytoplasmic matrix
We introduced several membrane-impermeant fluorescent dyes, including Lucifer Yellow, carboxyfluorescein, and fura-2, into the cytoplasmic matrix of J774 cells and thioglycollate-elicited mouse peritoneal macrophages by ATP permeabilization of the plasma membrane and observed the subsequent fate of these dyes. The dyes did not remain within the cytoplasmic matrix; instead they were sequestered ...
متن کاملMolecular architecture and the structural basis for anion interaction in prestin and SLC26 transporters
Prestin (SLC26A5) is a member of the SLC26/SulP anion transporter family. Its unique quasi-piezoelectric mechanical activity generates fast cellular motility of cochlear outer hair cells, a key process underlying active amplification in the mammalian ear. Despite its established physiological role, it is essentially unknown how prestin can generate mechanical force, since structural information...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016